The pomegranate (Punica granatum L.) is an indigenous minor fruit crop of Pakistan, having an extended historical past of cultivation as wild plant in warm temperate Himalayan vary of the rustic; then again its production is somewhat low to fulfill ever increasing market demand. Sustainable production of top of the range pomegranate fruit on the earth has been achieved through morpho-genetic characterization and estimation of germplasm variety which used to be lacking within the nation.
Thus you will need to estimate morphological, biochemical and genetic diversity of wild and domesticated pomegranate accessions through analysis of separate and combined data of selected wild and domesticated pomegranate accessions as a way to preserve the elite germplasm.
Various levels of qualitative and quantitative morphological range have been recorded in end result of decided on 115 pomegranate accessions. The spherical shape culmination were dominant in most of the commercial and wild accessions of Rawalakot whereas pink colour end result and arils had been main in wild compared to domesticated accessions.
The best possible price of CV was once estimated in seed hardness (302.91%) adopted by yellow green fruit colour (215.24%), round oblong end result (210.33%), quite sour arils (209.49%), red purple arils 177.33%) and white crimson arils (170.97%) in all decided on pomegranate accessions. The purple skin colour in fruit had a robust correlation (r=0.809) with crimson arils adopted by 0.769 and zero.741% for spherical fruit stalked end and medium seed hardness, respectively.
There used to be a powerful positive affiliation (0.731) of red arils with sourness and a damaging correlation (-0.145) with sweetness. The wild pomegranate accessions had high CV (29.07%), for fruit weight followed by 25.61, 24.73 and 20.45% for rind weight, rind thickness and seed weight, respectively, whereas, in domesticated accessions the highest CV of 41.99% used to be recorded in fruit weight. Fruit weight had a strong correlation (zero.79%) with rind weight and a prime positive correlation used to be recorded between wood portion index (WPI) and aril weight in a mixed analysis of untamed and domesticated accessions. All domesticated accessions had a detailed association amongst fruit weight, arils and seeds, while the wild accessions, there used to be a strong affiliation for WPI.
University: UNIVERSITY OF AGRICULTURE, FAISALABAD
Thesis link: http://prr.hec.gov.pk/jspui/handle/123456789//6993
The Abbottabad and Rawalakot accessions have been highly numerous and sundry now not most effective from each different but also from accessions from other regions, whereas accessions of Muzaffargarh and Rahim Yar Khan had prime similarities as shown in cluster research of qualitative data of all accessions. Wild and domesticated accessions effectively clustered at the basis of similarities of morphological and biochemical traits irrespective to rising areas; on the other hand, most of Muzaffargarh, Rahim Yar Khan and Mustong accessions grouped in the similar elegance. Moreover, wild and domesticated accessions grouped in separate classes for biochemical traits whilst a few Chakwal, Bahawalpur and D.G. Khan accessions clustered with wild accessions for most of the morphological characteristics.
Most of the reported SSR primers (29) on this learn about have been environment friendly and showed top polymorphism with polymorphic data contents (PIC) ranging from zero.187 to zero.5598 and most allele frequency of xviii zero.8579% in all selected wild and domesticated pomegranate accessions. The best genetic similarity coefficient used to be 30.7 to 84.76%, 21.76 to 76.78% and 21.76 to 79.88% in wild, domesticated and each wild and domesticated pomegranate accessions, respectively.
The domesticated accessions clustered on the foundation of genetic similarity regardless of growing areas while wild accessions sharing not unusual alleles were grouped in the similar elegance in response to growing areas. Thus morphological characteristics showed high variety in wild accessions compared to domesticated pomegranates whereas, biochemical characteristics confirmed prime range in domesticated accessions.
In molecular research, the SSR primers may function possible markers for genetic range estimation in Pakistani pomegranates as they proved that the genetic base of our pomegranate germplasm is extensive. Molecular diversity used to be upper in wild accessions in comparison to domesticated pomegranates. These research supply elementary data for pomegranate breeding programs to broaden new cultivars to broader the harvest window of top of the range pomegranate culmination in Pakistan.